Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8330710 | International Journal of Biological Macromolecules | 2015 | 10 Pages |
Abstract
In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC). CHS was prepared from squid pens by alkaline N-deacetylation. HA and β-TCP were extracted from mussel shells using a microwave irradiation method. Two different composites were prepared by incorporating 50% (w/w) HA/(β-TCP) in CHS or CHC followed by lyophilization and cross-linking of composites by tripolyphosphate (TPP). The effect of different freezing temperatures of â20, â80 and â196 °C on the physicochemical characteristics of composites was investigated. A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS. Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated. The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties. The results of this study indicated that composites produced at â20 °C had higher mechanical properties and lower degradation rate compared with â80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
Keywords
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Authors
Amin Shavandi, Alaa El-Din A. Bekhit, M. Azam Ali, Zhifa Sun,