Isolation and characterization of collagen from the skin of Brama australis

Collagen was extracted from the skin of Brama australis, the fish from warm-water sea. The yield of collagen from skin of B. australis was about 1.5% on a wet weight basis of raw material. The isolated protein was confirmed as collagen by different physico-chemical techniques such as: FTIR, SDS-PAGE, and amino acid analysis. The denaturation temperature (Td) of obtained collagen was found to be 24 °C, what is promising as an advantage for cosmetic application. According to the electrophoretic pattern, collagen consisted of two different α-chains (α1 and α2) and was classified as type I collagen. Although Td of obtained collagen is higher than 20 °C it is still far from Td of mammalian collagen.