Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8330800 | International Journal of Biological Macromolecules | 2015 | 22 Pages |
Abstract
Collagen was extracted from the skin of Brama australis, the fish from warm-water sea. The yield of collagen from skin of B. australis was about 1.5% on a wet weight basis of raw material. The isolated protein was confirmed as collagen by different physico-chemical techniques such as: FTIR, SDS-PAGE, and amino acid analysis. The denaturation temperature (Td) of obtained collagen was found to be 24 °C, what is promising as an advantage for cosmetic application. According to the electrophoretic pattern, collagen consisted of two different α-chains (α1 and α2) and was classified as type I collagen. Although Td of obtained collagen is higher than 20 °C it is still far from Td of mammalian collagen.
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Authors
Alina Sionkowska, Justyna KozÅowska, MaÅgorzata Skorupska, Marta Michalska,