Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8331161 | International Journal of Biological Macromolecules | 2015 | 11 Pages |
Abstract
An exopolysaccharide (KNPS) of an average molecular weight â¼1.8 Ã 105 Da was isolated from the culture medium of Klebsiella pneumoniae PB12. Structural characterization of KNPS was carried out using sugar and methylation analysis, Smith degradation and 1D/2D NMR experiments. Sugar analysis showed that the KNPS composed of arabinose, galactose, 3-O-methyl-galctose and glucose in a molar ratio of nearly 4:3:1:1. The proposed repeating unit of the KNPS has a backbone chain consisting of two (1 â 6)-galactopyranosyl residues, two (1 â 5)-arabinofuranosyl residues, one (1 â 6)-glucopyranosyl residue and one (1 â 3)-arabinopyranosyl residue, out of which one (1 â 6)-galactopyranosyl residue was branched at O-2 position with a (1 â 2)-linked-galactopyranosyl residue terminated with non reducing arabinofuranosyl residue and one (1 â 5)-arabinofuranosyl residue branched at O-3 position with non reducing end 3-O-Me-galactopyranosyl residue. KNPS was found non-toxic toward human lymphocyte up to the dosage of 100 μg/ml. KNPS enhanced malondialdehyde (MDA), reactive oxygen species (ROS), and have the potential to alter the ratio of oxidized glutathione (GSSG) and reduced glutathione (GSH) levels in the cellular system.
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Authors
Amit K. Mandal, Ipsita K. Sen, Prasenjit Maity, Sourav Chattopadhyay, Ranadhir Chakraborty, Somenath Roy, Syed S. Islam,