Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8331976 | International Journal of Biological Macromolecules | 2015 | 8 Pages |
Abstract
Hymenaea courbaril var courbaril seed xyloglucan was efficiently extracted with 0.1 M NaCl, followed by ethanol precipitation (yield = 72 ± 5% w/w). Its amorphous structure was identified by the pattern of X-ray diffraction. The monosaccharide composition was determined by GC/MS analysis of the alditol acetates and showed the occurrence of glucose:xylose:galactose:arabinose (40:34:20:6). One-(1D) and two-dimensional-(2D) NMR spectra confirmed a central backbone composed by 4-linked β-glucose units partially branched at position 6 with non-reducing terminal units of α-xylose or β-galactose-(1â2)-α-xylose disaccharides. The xyloglucan solution was evaluated by dynamic light scattering and presents a polydisperse and practically neutral profile, and at 0.5 and 1.0% (w/v) the solutions behave as a viscoelastic fluid. The polysaccharide did not show significant antibacterial or hemolytic activities. Overall our results indicate that xyloglucan from H. courbaril is a promising polysaccharide for food and pharmaceutical industries.
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Authors
Isabel R.S. Arruda, Priscilla B.S. Albuquerque, Gustavo R.C. Santos, Alexandre G. Silva, Paulo A.S. Mourão, Maria T.S. Correia, António A. Vicente, Maria G. Carneiro-da-Cunha,