Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8333564 | International Journal of Biological Macromolecules | 2013 | 9 Pages |
Abstract
The cytotoxic activity of β-d-glucans isolated from Agaricus bisporus and Lactarius rufus fruiting bodies was evaluated on human hepatocellular carcinoma cells (HepG2). NMR and methylation analysis suggest that these β-d-glucans were composed of a linear (1â6)-linked and a branched (1â3), (1â6)-linked backbone, respectively. They both decreased cell viability at concentrations of up to 100 μg mLâ1, as shown by MTT assay. The amount of LDH released and the analysis of cell morphology corroborated these values and also showed that the β-d-glucan of L. rufus was more cytotoxic to HepG2 cells than that of A. bisporus. The treatment of HepG2 cells with L. rufus and A. bisporus β-d-glucans at a dose of 200 μg mLâ1 for 24 h promoted an increase of cytochrome c release and a decrease of ATP content, suggesting that these polysaccharides could promote cell death by apoptosis. Both β-d-glucans were tested against murine primary hepatocytes at a dose of 200 μg mLâ1. The results suggest that the L. rufus β-d-glucan was as cytotoxic for hepatocytes as for HepG2 cells, whereas the A. bisporus β-d-glucan, under the same conditions, was cytotoxic only for HepG2 cells, suggesting cell selectivity. These results open new possibilities for use of mushroom β-d-glucans in cancer therapy.
Keywords
3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromideHEPESLactarius rufusASAAgaricus bisporusTRIS4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acidBSADMSOHCCMTTbovine serum albuminacetylsalicylic acidTris(hydroxymethyl)-aminomethaneDimethylsulfoxideHepG2 cellsCytotoxicitylactate dehydrogenaseLDHHepatocellular carcinoma
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Authors
Amanda do Rocio Andrade Pires, Andrea Caroline Ruthes, Silvia Maria Suter Correia Cadena, Alexandra Acco, Philip Albert James Gorin, Marcello Iacomini,