Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8334058 | International Journal of Biological Macromolecules | 2013 | 8 Pages |
Abstract
Pear-derived Weissella sp. TN610 produced extracellular glycosyltransferase activity responsible for the synthesis of soluble exopolysaccharide from sucrose. Acid and dextranase-catalyzed hydrolysis revealed that the synthesized polymer was a glucan. According to 1H and 13C NMR analysis, the glucan produced by TN610 was a linear dextran made of 96% α-(1â6) and 4% α-(1â3) linkages. Zymogram analysis confirmed the presence of a unique glucansucrase of approximately 180 kDa in the cell-free supernatant from TN610. The crude enzyme, optimally active at 37 °C and pH 5, has promising potential for application as a food additive since it catalyzes dextran synthesis in sucrose-supplemented milk, allowing its solidification. A 4257-bp product corresponding to the mature glucansucrase gene was amplified by PCR from TN610. It encoded a polypeptide of 1418 residues having a calculated molecular mass of 156.089 kDa and exhibiting 96% and 95% identity with glucansucrases from Lactobacillus fermentum Kg3 and Weissella cibaria CMU, respectively.
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Authors
Wacim Bejar, Valérie Gabriel, Myriam Amari, Sandrine Morel, Monia Mezghani, Emmanuelle Maguin, Catherine Fontagné-Faucher, Samir Bejar, Hichem Chouayekh,