Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8334112 | International Journal of Biological Macromolecules | 2012 | 8 Pages |
Abstract
Lipid-A was isolated by mild acid hydrolysis from lipopolysaccharides extracted from cells of Herbaspirillum seropedicae, strain SMR1, and from two mutants deficient in the biosynthesis of rhamnose (rmlBâ and rmlCâ). Structural analyzes were carried out using MALDI-TOF and derivatization by per-O-trimethylsilylation followed by GC-MS in order to determine monosaccharide and fatty acid composition. De-O-acylation was also performed to determine the presence of N-linked fatty acids. Lipid-A from H. seropedicae SMR1 showed a major structure comprising 2-amino-2-deoxy-glucopyranose-(1 â 6)-2-amino-2-deoxy-glucopyranose phosphorylated at C4â² and C1 positions, each carrying a unit of 4-amino-4-deoxy-arabinose. C2 and C2â² positions were substituted by amide-linked 3-hydroxy-dodecanoic acids. Both rhamnose-defective mutants showed similar structure for their lipid-A moieties, except for the lack of 4-amino-4-deoxy-arabinose units attached to phosphoryl groups.
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Authors
Rodrigo V. Serrato, Eduardo Balsanelli, Guilherme L. Sassaki, Russell W. Carlson, Artur Muszynski, Rose A. Monteiro, Fábio O. Pedrosa, Emanuel M. Souza, Marcello Iacomini,