| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8334132 | International Journal of Biological Macromolecules | 2013 | 7 Pages |
Abstract
In this work, the assembly of purified Bacillus subtilis FtsA was analyzed by several complimentary techniques. FtsA assembled to form filaments and bundles and the polymers disassembled upon dilution. FtsA assembled more efficiently at pH 6.0 as compared to that at pH 7.0 or 8.0 and high salt inhibited the assembly of FtsA. FtsA was found to hydrolyze ATP in vitro; however, neither ATP nor ADP influenced the assembly kinetics of FtsA. Though FtsA is a homologue of actin, cytochalasin D did not inhibit the assembly of FtsA. Interestingly, a hydrophobic molecule, 4,4â²-dianilino-1,1â²-binaphthyl-5,5â²-disulfonic acid, inhibited the assembly of FtsA.
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Authors
Parminder Singh, Ravindra D. Makde, Saikat Ghosh, Jayant Asthana, Vinay Kumar, Dulal Panda,