Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8334161 | International Journal of Biological Macromolecules | 2013 | 6 Pages |
Abstract
Polyphenolic-polysaccharide-protein complex has been isolated from flowers of Solidago canadensis L. by hot alkaline extraction procedure. Compositional analyses of S canadensis complex revealed the presence of carbohydrates (43Â wt%), protein (27Â wt%), phenolics (12Â wt%), uronic acids (10Â wt%) and inorganic material (8Â wt%). The carbohydrate part was rich in neutral sugars (81Â wt%) while uronids were determined in lower amount (19Â wt%). Monosaccharide analysis of carbohydrate part revealed the presence of five main sugar components, i.e. rhamnose (â¼23Â wt%), arabinose (â¼20Â wt%), uronic acids (â¼19Â wt%), galactose (â¼17Â wt%) and glucose (â¼14Â wt%), and indicated thus the presence of rhamnogalacturonan and arabinogalactan in S. canadensis complex. HPLC analysis of complex showed one single peak of molecule mass at 11.2Â kDa. Antitussive activity tests, performed in three doses of Solidago complex, showed the reduction of the number of cough efforts in the dose-dependent manner. Higher doses (50 and 75Â mg/kg b.w.) were shown to be by 15 and 20% more effective than that of lower one (25Â mg/kg b.w.). However, the antitussive effect of the highest dose (75Â mg/kg b.w.) was by 10% lower in comparison with that of codeine, the strongest antitussive agent. Besides, the highest dose of the complex (75Â mg/kg b.w.) significantly decreased values of specific airways resistance and their effect remained longer as that of salbutamol, a representative of classic antiasthmatic drugs.
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Authors
M. Å utovská, P. Capek, M. Kocmálová, S. FraÅová, I. Pawlaczyk, R. Gancarz,