| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8334331 | International Journal of Biological Macromolecules | 2012 | 6 Pages |
Abstract
⺠Preparation conditions of glycyrrhetinic acid liposome (GAL) were optimized by RSM. ⺠The optimal preparation conditions for GAL was ratio 9:1, soybean phospholipid cholesterol (w/w) 2.5:1 and water bath temperature 31 °C. ⺠GAL could significantly promote T and B lymphocytes proliferation and increase the concentration of IgG and IgM in vitro.
Keywords
immunoglobulins GPBSIgMLPSIgGCMFBBDPHAPhytohemagglutinin3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideDMSOMTTenzyme linked immunosorbent assayGlycyrrhetinic acidimmunoglobulinscalcium and magnesium-freeELISALymphocyte proliferationDimethyl sulfoxideEntrapment efficiencyResponse surface methodologyRSMBox–Behnken designPhosphate buffered salinelipopolysaccharideblank controlGal
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Authors
Xiaojuan Zhao, Jiaguo Liu, Yuanliang Hu, Yunpeng Fan, Deyun Wang, Ju Yuan, Liu Xu, Liu Cui, Zhang Jing,