Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8334582 | International Journal of Biological Macromolecules | 2012 | 4 Pages |
Abstract
Clanis bilineata larvae are widely consumed in China. In this study, chitooligosaccharides were prepared from C. bilineata larvae skin by demineralisation, deproteination, washing, drying, deacetylation, hydrolysis using commercial α-amylase, filtration, setting the preparation at approximately 15% (w/v), precipitation with 6 volumes of ethanol, and drying at 60 °C for 2 h. The optimal hydrolysis conditions were determined as follows: pH 5.5; temperature, 55 °C; enzyme amount, 40 mg/(g chitosan); reaction time, 4 h. The Fourier transform infrared spectra revealed that chitooligosaccharides with a degree of polymerisation in the range of 2-8 were the main component of the resulting product, with the chitooligosaccharide content and yield being 95.8% and 96.2% (w/w), respectively. The resulting product showed high antibacterial activity compared with the original chitosan.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Shengjun Wu,