Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8335458 | International Journal of Biological Macromolecules | 2011 | 6 Pages |
Abstract
ABPS-1, a new water-soluble polysaccharide with molecular weight of 26 kDa and a specific optical rotation of +170° (c 1.0, H2O), was extracted from the roots of Acanthophyllum bracteatum by warm water and further successively purified through DEAE-cellulose A52 and Sephadex G-100 columns. Monosaccharide analysis revealed that the ABPS-1 was composed of Glc, Gal and Ara with a relative molar ratio of 1.4:5.2:1.0. Its structural features were elucidated by a combination of FT-IR, methylation and GC-MS analysis, periodate oxidation and Smith degradation, partial acid hydrolysis and 13C and 1H NMR spectroscopy. The data obtained indicate that ABPS-1 possessed a backbone of α-(1 â 6)-linked Gal with branches attached to O-2 by α-1 â linked Glc and at O-3 by α-1 â linked Gal and by α-(1 â 3)-linked Ara. The in vitro antioxidant activity showed that ABPS-1 possesses DPPH radical-scavenging activity in a concentration-dependent manner with an EC50 value of 2.6 mg/ml.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Kambiz Jahanbin, Ahmad Reza Gohari, Sohrab Moini, Zahra Emam-Djomeh, Paolo Masi,