Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8335584 | International Journal of Biological Macromolecules | 2011 | 5 Pages |
Abstract
One water-soluble polysaccharide (ABP-W1) was purified from the fruiting bodies of Agaricus blazei by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. Its molecular weight was about 3.9 Ã 102 kDa as determined by high-performance size-exclusion chromatography (HPSEC). The structural feature of ABP-W1 was investigated by a combination of chemical and instrumental analysis, including partial hydrolysis with acid, periodate oxidation-Smith degradation, acetylation, methylation analysis and nuclear magnetic resonance spectroscopy (NMR 1H, 13C). The results revealed that ABP-W1 had a backbone consisting of (1 â 6)-linked-α-d-galactopyranosyl and (1 â 2,6)-linked-α-d-glucopyranosyl, which was branched with one single terminal (1â)-α-d-glucopyranosyl at the O-2 position of (1 â 2,6)-linked-α-d-glucopyranosyl along the main chain in the ratio of 1:1:1. The observation of the complex-formation between ABP-W1 and Congo Red indicated that ABP-W1 probably existed in a triple-strand helical conformation in water. Based on the data obtained, ABP-W1 was composed of a repeating unit with a structure as below:
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Authors
Jicheng Liu, Chunjing Zhang, Yajun Wang, Haitao Yu, Han Liu, Liping Wang, Xiuzhen Yang, Zhecheng Liu, Xianchun Wen, Yongxu Sun, Chunlei Yu, Lei Liu,