Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8336944 | The Journal of Nutritional Biochemistry | 2015 | 10 Pages |
Abstract
The objective of this study was to determine the extent to which a low level of trans-10, cis-12 (10,12) conjugated linoleic acid (CLA) decreases adiposity and increases browning in overweight mice, its dependence on inflammatory signaling and potential synergistic effects of daily exercise. Young, Sv129 male mice were fed a high-fat diet for 5 weeks to make them fat and glucose intolerant and then switch them to a low-fat diet with or without 0.1% 10,12 CLA, sodium salicylate or exercise for another 7 weeks. 10,12 CLA decreased white adipose tissue (WAT) and brown adipose tissue mass, and increased the messenger RNA and protein levels, and activities of enzymes associated with thermogenesis or fatty acid oxidation in WAT. Mice fed 10,12 CLA had lower body temperatures compared to controls during cold exposure, which coincided with decreased adiposity. Although sodium salicylate decreased 10,12 CLA-mediated increases in markers of inflammation in WAT, it did not affect other outcomes. Exercise had no further effect on the outcomes measured. Collectively, these data indicate that 10,12 CLA-mediated reduction of adiposity is independent of inflammatory signaling, and possibly due to up-regulation of fatty acid oxidation and heat production in order to regulate body temperature. Although this low level of 10,12 CLA reduced adiposity in overweight mice, hepatomegaly and inflammation are major health concerns.
Keywords
HLSNF-κBPPARELOVL3MCPCPTGPRIGTTBATTNFDEXAUCPCOX-2ERKAUCintraperitoneal glucose tolerance testDual-energy X-rayinflammationFatty acid oxidationinterleukinAdipose tissueWhite adipose tissuebrown adipose tissueThermogenesistriglycerideHADsodium salicylateCyclooxygenase-2endoplasmic reticulumtumor necrosis factornuclear factor kappa BObesityarea under the curveHLEexerciseUncoupling proteinmonocyte chemoattractant proteinWATCarnitine palmitoyltransferaseCideaextracellular signal-regulated kinaseperoxisome proliferator-activated receptor
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Wan Shen, Jessie Baldwin, Brian Collins, Lindsay Hixson, Kuan-Ting Lee, Timothy Herberg, Joseph Starnes, Paula Cooney, Chia-Chi Chuang, Robin Hopkins, Tanya Reid, Sat Gupta, Michael McIntosh,