Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8337822 | The Journal of Steroid Biochemistry and Molecular Biology | 2018 | 9 Pages |
Abstract
Historically sulfonated steroids were primarily considered as inactive metabolites destined for elimination. However, more recently they have been increasingly recognized as precursors for the production of bioactive steroids in target tissues and as functional molecules without preceding hydrolysis. In order to comprehensively characterize their occurrence in cyclic cows and their formation and hydrolysis in bovine ovarian steroidogenesis, ovaries from cyclic cows were screened for the expression of oestrogen sulfotransferase (SULTE1) and steroid sulfatase (STS) by Western blot and immunohistochemistry. Moreover, a broad spectrum of 13 sulfonated steroids was measured applying liquid chromatography-tandem mass spectrometry (LC-MS/MS) in blood samples collected from three cycling heifers during defined stages of the ovarian cycle and in fluid obtained from ovarian follicles of different size. SULT1E1 was undetectable in ovarian tissues. For STS only a weak immunostaining was found predominantly in granulosa cells of larger follicles. However, no specific band occurred in Western blot. In blood, concentrations of all sulfonated steroids investigated were below the limit of quantification (LOQ). In follicular fluid, only cholesterol sulfate was measured in considerable concentrations (328.3â¯Â±â¯63.8â¯ng/ml). However, the role of cholesterol sulfate in bovine follicular steroidogenesis remains unclear as concentrations were obviously unrelated to follicular size. The remaining sulfonated steroids investigated were undetectable or only slightly exceeded LOQ in a minor proportion of samples. The results are clearly contrary to a role of sulfonated steroids as important precursors, intermediates or products of bovine ovarian steroidogenesis.
Keywords
ESIepiandrosterone sulfate17α-hydroxypregnenolone17OHP4DHEADHTsSULT1E1CholSE1SSULTE2SIVMDHEASRIAdehydroepiandrosteroneIRSSDSIgGLOQAPCICOCsETsSTSPBST17α-HydroxyprogesteroneGC/MSLC–MS/MSAndrosteroneAndrosterone sulfateOestradiol-17βSteroid sulfataseandrostenedioneimmunoglobulin GIn vitro maturationOvarytestosteronecorpus luteumpregnenoloneradioimmunoassayANSsodium dodecyl sulfatedehydroepiandrosterone sulfatecytosolic sulfotransferasepregnenolone sulfateLiquid chromatography-tandem mass spectrometryFollicular fluidlimit of quantificationimmunoreactive scoreProgesteronecholesterol sulfateGas Chromatography-Mass SpectrometryBovineelectrospray ionizationatmospheric pressure chemical ionization
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Carina Blaschka, Gerhard Schuler, Alberto Sánchez-Guijo, Bettina Zimmer, Sabine Feller, Franziska Kotarski, Stefan A. Wudy, Christine Wrenzycki,