| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8339001 | The Journal of Steroid Biochemistry and Molecular Biology | 2013 | 9 Pages |
Abstract
- Androgen deprivation therapy (ADT) is an effective treatment for prostate cancer.
- We present a method for quantification of free and conjugated keto-androgens using SID-LC/ESI/SRM/MS.
- This method was validated using standards set by the United States Food and Drug Administration (US FDA).
- This was applied to patients enrolled in the Targeted Androgen Pathway Suppression (TAPS) clinical trial.
- The method can be used to interrogate personalized response and resistance to ADT.
Keywords
ADTCRPCDHEA-SCD-FBSTAPSRIAAdionedehydroepiandrosteroneHSDTFAUGTDHTLLOQPSAGnRHDHEA5α-androstane-3,17-dione5α-Dihydrotestosteronem/zUDP glucuronosyltransferaseProstate specific antigenAndrosteroneTrifluoroacetic acidEpitestosteroneEnzyme-linked immunosorbent assayELISAtestosteronenot detectedlower limit of quantificationandrogen deprivation therapyradioimmunoassayProstate cancerCastration resistant prostate cancerdehydroepiandrosterone sulfateCoefficient of VariationMass spectrometrynot significantmass to charge ratiogonadotropin releasing hormonehydroxysteroid dehydrogenaseliquid chromatographyquality control
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Authors
Daniel Tamae, Michael Byrns, Brett Marck, Elahe A. Mostaghel, Peter S. Nelson, Paul Lange, Daniel Lin, Mary-Ellen Taplin, Steven Balk, William Ellis, Larry True, Robert Vessella, Bruce Montgomery, Ian A. Blair, Trevor M. Penning,