Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8339022 | The Journal of Steroid Biochemistry and Molecular Biology | 2013 | 9 Pages |
Abstract
The aim of this study was to investigate the effect of granulosa cells from small antral follicles on steroidogenesis, proliferation and apoptosis of goat ovarian stromal and theca cells in vitro. Using Transwell co-culture system, we evaluated androgen production, LH responsiveness, cell proliferation and apoptosis and some molecular expression regarding steroidogenic enzyme and apoptosis-related genes in stromal and theca cells. The results indicated that the co-culture with granulosa cells increased steroidogenesis, LH responsiveness and bcl-2 gene expression as well as decreased apoptotic bax and bad expressions in stromal and theca cells. Thus, granulosa cells had a capacity of promoting steroidogenesis in stromal cell and LH responsiveness in cortical stromal cells, maintaining steroidogenesis in theca cells, inhibiting apoptosis of cortical stromal cells and improving anti-apoptotic abilities of stromal and theca cells.
Keywords
TCSGAPDHBcl-2SCsCholesterol side-chain cleavage cytochrome P450LHRCYP11A1CYP17A1GCsHSD3B1SteroidogenesisBaxBADGoatCell proliferationApoptosisStarTheca cellsgranulosa cellsBcl-2-associated X proteinSteroidogenic acute regulatory proteinSMSglyceraldehyde-3-phosphate dehydrogenaseLuteinizing hormone receptor
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Authors
Mingning Qiu, Fusheng Quan, Chengquan Han, Bin Wu, Jun Liu, Zhongcai Yang, Feng Su, Yong Zhang,