| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8340108 | Methods | 2018 | 18 Pages |
Abstract
To illustrate how QP-DHM can reveal the dynamics of specific cellular processes, we show how the monitoring of transmembrane water movements can be used to resolve the neuronal network dynamics at single-cell level. This is possible because QPS can measure the activity of electroneutral cotransports, including NKCC1 and KCC2, during a neuronal firing mediated by glutamate, the main excitatory neurotransmitter in the brain. Finally, we added a supplemental section, with more technical details, for readers who are interested in troubleshooting live-cell QP-DHM.
Keywords
FOVμLμMdeoxyribonucleaseAMPANMDAN-methyl-d-aspartateKCC2MK-801CCDMAP-2NKCC1D-PBSOPLQPSQPImOsmFemtoliterE17DHMHEPESGFAPEtOH4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid6-Cyano-7-nitroquinoxaline-2,3-dioneDNAseEDTAEthylenediaminetetraacetic acidγ-aminobutyric acidTemperature differencedry massCharge-Coupled DeviceBiphasicnumerical apertureHourCNQXCMOSRefractive indexOptical path lengthDulbecco's phosphate-buffered salineField of viewMicrolitermicromolarNeuronsGlial fibrillary acidic proteinmicrotubule-associated protein 2Kramers-KronigGABA
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Authors
Sébastien A. Lévesque, Jean-Michel Mugnes, Erik Bélanger, Pierre Marquet,