Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8340278 | Methods | 2016 | 11 Pages |
Abstract
The importance of elucidating the three dimensional structures of RNA molecules is becoming increasingly clear. However, traditional protein structural techniques such as NMR and X-ray crystallography have several important drawbacks when probing long RNA molecules. Single molecule Förster resonance energy transfer (smFRET) has emerged as a useful alternative as it allows native sequences to be probed in physiological conditions and allows multiple conformations to be probed simultaneously. This review serves to describe the method of generating a three dimensional RNA structure from smFRET data from the biochemical probing of the secondary structure to the computational refinement of the final model.
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Authors
James D. Stephenson, Julia C. Kenyon, Martyn F. Symmons, Andrew M.L. Lever,