Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8340303 | Methods | 2016 | 8 Pages |
Abstract
Transplantation of vascular smooth muscle cells (VSMCs) is a promising cellular therapy to promote angiogenesis and wound healing. However, VSMCs are derived from diverse embryonic sources which may influence their role in the development of vascular disease and in its therapeutic modulation. Despite progress in understanding the mechanisms of VSMC differentiation, there remains a shortage of robust methods for generating lineage-specific VSMCs from pluripotent and adult stem/progenitor cells in serum-free conditions. Here we describe a method for differentiating pluripotent stem cells, such as embryonic and induced pluripotent stem cells, as well as skin-derived precursors, into lateral plate-derived VSMCs including 'coronary-like' VSMCs and neural crest-derived VSMC, respectively. We believe this approach will have broad applications in modeling origin-specific disease vulnerability and in developing personalized cell-based vascular grafts for regenerative medicine.
Keywords
HPSCsVEGFR2qRT-PCRSRFIL-1βIPSCDKK1IMDMSKPASMAEBsVSMCsPDGFRαKDRBrachyuryHASMCTIMPsFLK1TGF-βmonothioglycerolbFGFMTGMmpsFITCPAHalpha smooth muscle actinIscove’s Modified Dulbecco’s Mediuminterleukin 1 betaSerum-freeTransforming Growth Factor Betaembryoid bodiesESChuman aortic smooth muscle cellVascular smooth muscle cellEmbryonic stem cellInduced pluripotent stem cellsInduced pluripotent stem cellHuman pluripotent stem cellsVascular smooth muscle cellsEmbryonic stem cellsVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)basic fibroblast growth factorserum response factorPulmonary arterial hypertensionfluorescein isothiocyanateneural crestMatrix metalloproteinasesBMPLateral plate mesodermTissue inhibitor of metalloproteinasesquantitative real-time polymerase chain reactionBone morphogenetic proteinplatelet derived growth factor receptor alphaCoronaryfetal liver kinase 1kinase insert domain receptorvascular endothelial growth factor receptor 2
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Authors
Sarah K. Steinbach, Mansoor Husain,