Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8340367 | Methods | 2016 | 8 Pages |
Abstract
A number of non-membranous cellular bodies have been identified in higher eukaryotes, and these bodies contain a specific set of proteins and RNAs that are used to fulfill their functions. The size of these RNA-containing cellular bodies is usually on a submicron scale, making it difficult to observe fine structures using optical microscopy due to the diffraction limitation of visible light. Recently, microscope companies have released super-resolution microscopes that were developed using different principles, enabling the observation of sub-micron structures not resolvable in conventional fluorescent microscopy. Here, we describe multi-color fluorescent in situ hybridization techniques optimized for the simultaneous detection of RNA and proteins using super-resolution microscopy, namely structured illumination microscopy (SIM).
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Authors
Mari Mito, Tetsuya Kawaguchi, Tetsuro Hirose, Shinichi Nakagawa,