Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8340448 | Methods | 2016 | 23 Pages |
Abstract
In past decades, the experimental determination of protein functions was expensive and time-consuming, so numerous computational methods were developed to speed up and guide the process. However, most of these methods predict protein functions at the gene level and do not consider the fact that protein isoforms (translated from alternatively spliced transcripts), not genes, are the actual function carriers. Now, high-throughput RNA-seq technology is providing unprecedented opportunities to unravel protein functions at the isoform level. In this article, we review recent progress in the high-resolution functional annotations of protein isoforms, focusing on two methods developed by the authors. Both methods can integrate multiple RNA-seq datasets for comprehensively characterizing functions of protein isoforms.
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Authors
Wenyuan Li, Chun-Chi Liu, Shuli Kang, Jian-Rong Li, Yu-Ting Tseng, Xianghong Jasmine Zhou,