| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8340494 | Methods | 2015 | 27 Pages |
Abstract
The results demonstrate the advantages of the photo-initiated crosslinking technique which is able to map the protein-protein interfaces not only in the solvent exposed regions, but also in the membrane-embedded segments (compared to a typical crosslinking approach which generally only identifies crosslinks in solvent exposed regions).
Keywords
TFAa.i.PMETPRODDilauroylphosphatidylcholineMutMFTICRDLPCMFOP450E. coliNADPHIPTGMALDI-TOFSECEDCMS/MSSDS–PAGEtris(2-carboxyethyl)phosphineTrifluoroacetic acidEscherichia coliSodium dodecyl sulfate polyacrylamide gel electrophoresisisopropyl β-D-1-thiogalactopyranosideProtein–protein interactionMindaltonminuteSecondFourier transform ion cyclotron resonanceHourTCEPcytochrome P-450Absolute intensityMass spectrometryTandem mass spectrometrylysogeny brothMethionineMETwild typenicotinamide adenine dinucleotide phosphate (reduced)polymerase chain reactionPCRMolecular weightliquid chromatographyhigh-performance liquid chromatographyHPLC
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Authors
TomáÅ¡ JeÄmen, Renata PtáÄková, VÄra Äerná, Helena DraÄÃnská, Petr Hodek, Marie Stiborová, JiÅà HudeÄek, Miroslav Å ulc,