| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8340511 | Methods | 2015 | 10 Pages |
Abstract
This review explains the basic principles behind STED microscopy and the differences with other super-resolution techniques. It provides practical information on how to construct and operate a STED microscope that can be used for nanoscale imaging of GFP and its variants in living brain slices. We conclude by highlighting a series of recent technological innovations that are bound to enhance its scope and performance in the near future.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Ronan Chéreau, Jan Tønnesen, U. Valentin Nägerl,