Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8340514 | Methods | 2015 | 5 Pages |
Abstract
Although considerable progress has been made in imaging distances in cells below the diffraction limit using FRET and super-resolution microscopy, methods for determining the separation of macromolecules in the 10-50Â nm range have been elusive. We have developed fluorophore localisation imaging with photobleaching (FLImP), based on the quantised bleaching of individual protein-bound dye molecules, to quantitate the molecular separations in oligomers and nanoscale clusters. We demonstrate the benefits of using our method in studying the nanometric organisation of the epidermal growth factor receptor in cells.
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Authors
Stephen E.D. Webb, Michael Hirsch, Sarah R. Needham, Benjamin C. Coles, Kathrin M. Scherer, Selene K. Roberts, Laura C. Zanetti-Domingues, Christopher J. Tynan, Marisa L. Martin-Fernandez, Daniel J. Rolfe,