Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8340675 | Methods | 2015 | 9 Pages |
Abstract
This article discusses the issues to consider in the development and implementation of high-throughput screens (HTSs) using both siRNA libraries and small molecule compound collections, in order to discover autophagy regulators in mammalian cells. We discuss how to develop the screen, focusing on the key parameters to establish in order to perform a successful screen. As our understanding of autophagy increases and its impact on human disease is elucidated, this technology can be further exploited to uncover novel genes, which may one day become new therapeutic targets.
Keywords
AutophagosomeBAFAubiquitin-associatedUBAGFP-LC3EBSSp62ATGMADCRISPRshRNAHTSautophagy relatedSmall interfering RNAshort hairpin RNAsiRNAz-scoreAutophagybafilomycin A1LIRclustered regularly interspaced short palindromic repeatsEarle’s Balanced Salt Solutionhigh-throughput screenshigh-throughput screenphosphatidylethanolamineLC3-interacting regionmedian absolute deviationAmino acid starvation
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Authors
Justin Joachim, Ming Jiang, Nicole C. McKnight, Michael Howell, Sharon A. Tooze,