Post-transcriptional regulation of the human inducible nitric oxide synthase (iNOS) expression by the cytosolic poly(A)-binding protein (PABP)

Article ID	Journal	Published Year	Pages	File Type
8346092	Nitric Oxide	2013	12 Pages	PDF

Abstract

In summary, our data demonstrate that PABP by binding to specific sequence elements in the 5â²-UTR post-transcriptionally enhances human iNOS mRNA stability and thereby iNOS expression.

Keywords

β-tubulin ELAV β2M hnRNP YB-1 IFN-γ DRB TTP IL-1β iNOS qRT-PCR AUF1 human antigen R GAPDH LUC inducible NO-synthase FACS KH-type splicing regulatory protein ARE-BP eRF3 PABP eGFP KSRP shRNA GST 3′-UTR 3′-untranslated region 5′-UTR Small interfering RNA small hairpin RNA siRNA β2-microglobulin interferon-γ Interleukin-1β Immunoprecipitation Tristetraprolin coding sequence tumor necrosis factor-α mRNA stability fluorescent activated cell sorting embryonic lethal abnormal vision Control cells AU-rich element TNF-α luciferase cytokine mixture 5′-untranslated region UTR یا untranslated regions Nitric oxide ARE quantitative reverse transcription polymerase chain reaction Western blot enhanced green fluorescence protein HuR CdS glutathione S-transferase glyceraldehyde-3-phosphate dehydrogenase

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

Post-transcriptional regulation of the human inducible nitric oxide synthase (iNOS) expression by the cytosolic poly(A)-binding protein (PABP)

Authors

Ingrid Casper, Sebastian Nowag, Kathrin Koch, Thomas Hubrich, Franziska Bollmann, Jenny Henke, Katja Schmitz, Hartmut Kleinert, Andrea Pautz,