Article ID Journal Published Year Pages File Type
8346167 Nitric Oxide 2013 8 Pages PDF
Abstract
► We investigated the kinetics of DAF-FM activation by NO in aerated solutions. ► Data suggest NO2 rather than N2O3 as the reactive NO oxidation intermediate. ► The slope of DAF-T signal is related to [NO] according to ddt[FI]=2αk1[NO]2[O2][DAF-FM]k[NO]+[DAF-FM]. ► For [DAF-FM] > > [NO] the slope is proportional to [NO]2 and independent of [DAF-FM]. ► Recommendations for the calibration of DAF fluorescence are provided.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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