Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression

Article ID	Journal	Published Year	Pages	File Type
8359388	Protein Expression and Purification	2018	23 Pages	PDF

Keywords

CAT GFP IPTG Chaperone 2-ME 2-mercaptoethanol Autophosphorylation Solubility X-ray crystallography Tev Cell-free protein synthesis Matrix-assisted laser desorption/ionization-time of flight mass spectrometry MALDI-TOF MS Hck Tobacco etch virus green fluorescent protein chloramphenicol acetyl-transferase hematopoietic cell kinase

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

Preview

Phosphorylated and non-phosphorylated HCK kinase domains produced by cell-free protein expression

Authors

Kazushige Katsura, Yuri Tomabechi, Takayoshi Matsuda, Mayumi Yonemochi, Junko Mikuni, Noboru Ohsawa, Takaho Terada, Shigeyuki Yokoyama, Mutsuko Kukimoto-Niino, Chie Takemoto, Mikako Shirouzu,