| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8359840 | Protein Expression and Purification | 2016 | 9 Pages |
Abstract
In this work, transcription of pdh in A. bisporus was investigated with real-time PCR revealing influence of the carbon source on pdh expression levels. The gene was isolated and heterologously expressed in Pichia pastoris. Characterization of the recombinant enzyme showed a higher affinity towards disaccharides compared to other tested pyranose dehydrogenases from related Agariceae. Homology modeling and sequence alignments indicated that two loops of high sequence variability at substrate access site could play an important role in modulating these substrate specificities.
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Authors
Christoph Gonaus, Roman Kittl, Christoph Sygmund, Dietmar Haltrich, Clemens Peterbauer,