| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8360409 | Protein Expression and Purification | 2015 | 6 Pages |
Abstract
The central composite design and the response surface analysis proved to be suitable tools for a quick and efficient study. As a result, the optimal extraction conditions in PEG3350/NaCit system were 3.34% wt/wt for NaCl concentration, a biomass load which represented 9.30% wt/wt of the total ATPS mass and 6.37 top/bottom volume ratio giving a purification factor of 2.55 and a yield of 99.7% in top phase.
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Authors
RocÃo L. Pérez, Dana B. Loureiro, Bibiana B. Nerli, Gisela Tubio,