Article ID Journal Published Year Pages File Type
8360591 Protein Expression and Purification 2014 6 Pages PDF
Abstract
A winged bean trypsin inhibitor (WbTI-2) of molecular mass ∼20 kDa, has been cloned and expressed in Escherichiacoli with full activity like the one from seed protein. It completely inhibits trypsin at an enzyme:inhibitor molar ratio of 1:2. PCR with cDNA and genomic DNA using same primers produced about 550 base pair product, which indicated it to be an intronless gene. Through site-directed mutagenesis, the Arg64 has been confirmed as the P1 residue. For the presence of five methionine residues in WbTI-2, cyanogen bromide (CNBr) digestion was carried out. Out of three fragments the one (about 65% of original size) containing the reactive site loop retained 50% activity.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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