| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8360787 | Protein Expression and Purification | 2011 | 6 Pages |
Abstract
⺠We develop a new strategy which improves purification of sumoylated proteins in E. Coli. ⺠Our three-step purification procedure can be applied to a wide variety of targets. ⺠We obtain pure SUMO-1 modified IκBα in milligrams quantities. ⺠High yield expression is suitable for structural studies.
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Authors
Zoé Lens, Frédérique Dewitte, Carine Van Lint, Yvan de Launoit, Vincent Villeret, Alexis Verger,