Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8360808 | Protein Expression and Purification | 2011 | 7 Pages |
Abstract
⺠TSR(2,3) expression in Escherishia coli as a GST fusion affords excellent yield and purity. ⺠Recombinant TSR(2,3) binds histidine-rich glycoprotein, a canonical TSP-1 ligand. ⺠Recombinant TSR(2,3) exhibits anti-chemotactic activity similar to full-length TSP-1. ⺠X-ray crystal structures of E. coli and Drosophila expressed TSR(2,3) are identical.
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Authors
Philip A. Klenotic, Richard C. Page, Saurav Misra, Roy L. Silverstein,