Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8360817 | Protein Expression and Purification | 2011 | 6 Pages |
Abstract
⺠Cationic cell wall peroxidase from poplar tree was expressed in Escherichia coli as an inclusion body. ⺠The optimal conditions for refolding CWPO_C were 100 mM Tris-HCl at pH 8.5, 0.6 mM GSSG, 5 μM hemin, 0.6 M GmdCl and 5 mM CaCl2. ⺠kcat/Km for sinapyl alcohol of CWPO_C was over 170 times higher than that of HRP_C. ⺠Refolded CWPO_C possesses a very unique property of S-peroxidase.
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Authors
Le Thanh Mai Pham, Su Jin Kim, Bong Keun Song, Yong Hwan Kim,