A biological study establishing the endotoxin limit for osteoblast and adipocyte differentiation of human mesenchymal stem cells

Since endotoxins can affect various cellular functions, an endotoxin limit should be established for in vitro MSC cultures. Its no-observed-adverse-effect level was 0.1 ng/ml based on the effect on the hMSC osteoblast differentiation, but it may not necessarily be the limit for ADSCs.