Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8420251 | Journal of Microbiological Methods | 2018 | 4 Pages |
Abstract
We evaluated three DNA extraction methods for confirmation of Mycobacterium avium subspecies paratuberculosis from liquid cultures of bovine feces. Use of DNA Extract All Reagents Kit⢠resulted in efficient extraction of amplifiable DNA from higher proportion (96.29%) of known positive samples compared to Chelex-100 resin (25.92%) and polyethylene glycol (0%).
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Authors
Nagaraja R. Thirumalapura, Willard Feria, Deepanker Tewari,