Article ID Journal Published Year Pages File Type
8420361 Journal of Microbiological Methods 2018 12 Pages PDF
Abstract
Bacillus subtilis pheS was genetically modified to obtain a counter-selection marker with high selection efficiency in Bacillus amyloliquefaciens. The application of the new replication-thermosensitive integrative vector pNZTM1, containing this marker, pheSBsT255S/A309G, with a two-step replacement recombination procedure provides an effective tool for the genetic engineering of industrially important Bacillus species.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
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Improving the selection efficiency of the counter-selection marker pheS* for the genetic engineering of Bacillus amyloliquefaciens
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