Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8420377 | Journal of Microbiological Methods | 2018 | 6 Pages |
Abstract
Performance of the in-house protocol was evaluated using a total of 117 monomicrobial cases of positive blood culture. Medium from blood culture bottles was pretreated by the in-house protocol or the commercial kit, and isolated cells were subjected to direct identification by mass spectrometry fingerprinting in parallel with conventional subculturing for reference identification. The overall MALDI-TOF MS-based identification rates with scoreâ¯>â¯1.7 andâ¯>â¯2.0 obtained using the in-house protocol were 99.2% and 85.5%, respectively, whereas those obtained using the Sepsityper Kit were 85.4% and 61.5%, respectively. For Gram-positive cases, the in-house protocol yielded scores >1.7 andâ¯>â¯2.0 at 98.5% and 76.1%, respectively, whereas the commercial kit yielded these scores at 76.1% and 43.3%, respectively. Although these are preliminary results, these values suggest that this easy lysis-filtration protocol deserves assessment in a larger-scale test.
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Authors
Sachio Tsuchida, Syota Murata, Akiko Miyabe, Mamoru Satoh, Masaki Takiwaki, Kazuyuki Matsushita, Fumio Nomura,