| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8420388 | Journal of Microbiological Methods | 2018 | 4 Pages |
Abstract
Aggregatibacter (A.) actinomycetemcomitans is a periodontopathogenic bacterium causing aggressive periodontitis. Here we describe a single-step transconjugation system as novel and easily applicable protocol for site-specific genetic manipulation of A. actinomycetemcomitans. Deletion of PgaC, which is involved in the synthesis of biofilm matrix, led to a reduced biofilm formation.
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Authors
Nico S. Stumpp, Sarah A. Konze, Rita Gerardy-Schahn, Meike Stiesch, Falk F.R. Buettner,