Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8420407 | Journal of Microbiological Methods | 2018 | 15 Pages |
Abstract
Guiana extended-spectrum (GES) β-lactamases are emerging in Japan. The GES family can be classified into 2 groups, one with extended-spectrum β-lactamase (ESBL)-like activity, which hydrolyzes penicillins and cephalosporins, and the other with carbapenemase-like activity with an extended spectrum toward carbapenems. This difference is mediated by variations in a specific amino acid in the GES protein: G170â¯N or G170S substitutions. We developed an amplification refractory mutation system (ARMS) PCR assay that enabled rapid identification of these variant genes without sequencing.
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Authors
Shizuo Kayama, Raita Yano, Katsutoshi Yamasaki, Chiemi Fukuda, Keiko Nishimura, Hitoshi Miyamoto, Hiroki Ohge, Motoyuki Sugai,