Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8421148 | Journal of Microbiological Methods | 2016 | 8 Pages |
Abstract
Beadbeating increases the protein and protease activity when extracting from a faecal sample, however, the extracted protein is not stable and activity is lost, even with a suitable storage buffer. The most robust solution is to store the proteins in an intact frozen native faecal matrix and extract at the time of assay or analysis, this approach was shown to be suitable for samples in which, there are low levels of protease activity and which had been frozen for a year.
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Authors
Laura S. Morris, Julian R. Marchesi,