| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8421417 | Journal of Microbiological Methods | 2015 | 5 Pages |
Abstract
We report the development and evaluation of a serotype-specific xTAG luminex assay (SSA) that allows detection of the 33 serotypes of Streptococcus suis (S. suis). This assay is based on wzy gene targets directly involved in the cps biosynthesis and can be completed 40Â min post-PCR amplification. The assay correctly and specifically identified the serotype of all 209 isolates tested, in comparison with two serotyping multiplex PCR methods previously developed. The sensitivity was higher than that of the previously described methods. The SSA system described here provides an easy-to-use, high-throughput system for rapid detection of S. suis serotypes.
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Authors
Xuemei Bai, Zhijie Liu, Shaobo Ji, Marcelo Gottschalk, Han Zheng, Jianguo Xu,