Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8421690 | Journal of Microbiological Methods | 2015 | 4 Pages |
Abstract
An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives. The method was successfully applied to a gfp-labeled Yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization of Steinernema nematodes hosting their Xenorhabdus symbiont using dual-color confocal microscopy.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Samuel Gengler, Henri Batoko, Pierre Wattiau,