Article ID Journal Published Year Pages File Type
8421690 Journal of Microbiological Methods 2015 4 Pages PDF
Abstract
An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives. The method was successfully applied to a gfp-labeled Yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization of Steinernema nematodes hosting their Xenorhabdus symbiont using dual-color confocal microscopy.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
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