| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8421766 | Journal of Microbiological Methods | 2014 | 7 Pages |
Abstract
Using combined confocal laser scanning and atomic force microscopy (CLSM/AFM), bacterial viability under organic solvent stress was assessed at single cell level. Solvent-exposed bacteria stained with the LIVE/DEAD BacLight fluoresced green or red, allowing viable and dead cell discrimination. However, with toluene, butanol and acetonitrile, dually fluorescent cells appeared having compromised cell membranes. Changes in size, surface/volume ratio and roughness were revealed as possible resistance mechanisms.
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Authors
Maria S. Kuyukina, Irena B. Ivshina, Irina O. Korshunova, Ekaterina V. Rubtsova,