Article ID Journal Published Year Pages File Type
8422017 Journal of Microbiological Methods 2014 4 Pages PDF
Abstract
The LE (LguI/Eco81I)-cloning procedure allows a step-wise, directional fusion of multiple DNA-fragments into a vector by utilizing two restriction enzymes generating identical non-palindromic overhangs. This strategy was applied to produce heat-stable cellulase-fusion proteins containing up to five single moieties. Terminal affinity tags enable efficient purification using a simple two-step approach.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
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