| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8422204 | Journal of Microbiological Methods | 2014 | 8 Pages |
Abstract
The genetic study of mycobacteria, such as Mycobacterium tuberculosis and Mycobacterium ulcerans, is hampered heavily by their slow growth. We have developed efficient, versatile, and improved genetic tools for constructing unmarked recombinant mycobacteria more rapidly including generating multiple mutants using the same antibiotic marker in both fast- and slow-growing mycobacteria.
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Authors
Feng Yang, Yaoju Tan, Jia Liu, Tianzhou Liu, Bangxing Wang, Yuanyuan Cao, Yue Qu, Trevor Lithgow, Shouyong Tan, Tianyu Zhang,