Article ID Journal Published Year Pages File Type
8422289 Journal of Microbiological Methods 2014 6 Pages PDF
Abstract
A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
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A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches
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