Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8422438 | Journal of Microbiological Methods | 2014 | 9 Pages |
Abstract
Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species.
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Authors
LukáÅ¡ Krásný, Eva Rohlová, Helena RůžiÄková, JiÅà ŠantrůÄek, Radovan Hynek, Igor Hochel,