Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8509121 | Bulletin of Faculty of Pharmacy, Cairo University | 2017 | 6 Pages |
Abstract
In present study HPTLC method was developed and validated for the determination of friedelin in Putranjiva roxburghii Wall (family: Euphorbiaceae) leaf, bark extract and in polyherbal formulations. Analysis of samples were performed on TLC aluminium precoated plate (60 F254) by using mobile phase toluene: chloroform (9:1 v/v). Plate was derivatized with vanillin sulphuric acid and scanned at 580 nm. Developed method found to give compact spot for friedelin at Rf value 0.43 ± 0.01. The method was validated using International Council for Harmonization (ICH) guidelines including linearity, precision, accuracy, and robustness. Friedelin was found to be present in leaf extract of Putranjiva roxburghii Wall (0.003% w/w), in bark (0.04% w/w), formulation 1 (0.002% w/w) and formulation 2 (0.035% w/w). A good linearity relationship was found to be (100-500 ng spotâ1) with correlation coefficient (r2) value of 0.9892 for friedelin. Limit of detection and limit of quantitation was found to be 32.15, 97.44 ng/band respectively for friedelin. The developed method was found to be accurate and precise with 0.78%, 0.9% (%RSD) for interday and intraday precision. Accuracy of the method was performed by recovery studies at three different concentration levels and the average percentage recovery was found to be 98.55% for friedelin. The proposed method for the quantitation of friedelin was found to be simple, specific, accurate and robust in Putranjiva roxburghii Wall and polyherbal formulations.
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Authors
Kedar Kalyani Abhimanyu, Chaudhari Sanjay Ravindra, Rao Srinivasa Avanapu,